Mammalian cells respond to their substrates by complex changes in gene expression
profiles, morphology, proliferation and migration. We report that substrate nanotopography
alters morpohology and proliferation of human embryonic stem cells (hESCs). Fibronectin-
coated poly (di-methyl siloxane) substrates with line-grating (600nm ridges with 600nm
spacing and 600±150nm feature height) induced hESC alignment and elongation, mediated
the organization of cytoskeletal components including actin, vimentin, and α-tubulin, and …